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Ozias-Akins Biotechnology Laboratory papers & presentations |
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Selected Papers and Presentations View selected papers and presentations listed on the NESPAL website. All Refereed Publications Akiyama, Y, S. Goel, Z. Chen, W.W. Hanna, and P. Ozias-Akins. 2006. Pennisetum squamulatum: Is the Predominant Cytotype Hexaploid or Octaploid? J. Hered. doi:10.1093/jhered/esl005 Gualtieri, G., J. A. Conner, D. T. Morishige, J. E. Mullet, and P. Ozias-Akins. 2006. A segment of the apospory-specific genomic region is highly microsyntenic not only between the apomicts Pennisetum squamulatum and buffelgrass, but also with a rice chromosome 11 centromeric-proximal genomic region. Plant Physiol. 140:963-971. Goel, S., Z. Chen, Y. Akiyama, J. A. Conner, M. Basu, G. Gualtieri, W. W. Hanna, P. Ozias-Akins. 2006. Comparative Physical Mapping of the Apospory-Specific Genomic Region in Two Apomictic Grasses, Pennisetum squamulatum and Cenchrus ciliaris. Genetics 173:389-400. Ramos, M.L., G. Fleming, Y. Chu, Y. Akiyama, M.Gallo, P. Ozias-Akins. 2006. Genomic and evolutionary context for conglutin genes in Arachis. Mol. Gen. Gen. 275:578-592. Ozias-Akins, P. 2006. Apomixis: Developmental Characteristics and Genetics. Crit. Rev. Plant Sci. 25:199-214. Ozias-Akins, P. 2005. Peanut. In: Biotechnology in Agriculture and Forestry – Tropical Crops I. E.C. Pua and M.R. Davey, eds. Springer, Heidelberg. Akiyama, Y., W.W. Hanna, P. Ozias-Akins. 2005. High-resolution mapping reveals that the apospory-specific genomic region (ASGR) in C. ciliaris is located on a heterochromatic and hemizygous region of a single chromosome. Theor. Appl. Genet. 111:1042-1051. Joshi, M., C. Niu, G. Fleming, S. Hazra, Y. Chu, C. J. Nairn, H. Yang, P. Ozias-Akins. 2005. Use of green fluorescent protein as a non-destructive marker for peanut genetic transformation . In Vitro Plant 41:437-445. Sakhanokho, H.F., P. Ozias-Akins, O.L. May, and P.W. Chee. 2005. Putrescine enhances somatic embryogenesis and plant regeneration in elite upland cotton (Gossypium hirsutum L.) genotypes. Plant Cell Tiss. Organ Cult. 81:91-95. Jauhar, P.P., K.N. Rai, P. Ozias-Akins, Z. Chen, and W.W. Hanna. 2005. Genetic Improvement of Pearl Millet for Grain and Forage Production: Cytogenetic Manipulation and Heterosis Breeding. In: R.J. Singh and P.P. Jauhar, eds., Genetic Resources, Chromosome Engineering and Crop Improvement, CRC Taylor and Francis, pp 281-307. Goldman, J. W.W. Hanna, and P. Ozias-Akins. 2004. Tissue culture, plant regeneration and an analysis of somaclonal variation from TifEagle and TifSport bermudagrass cultivars. HortScience 39:1381-1384. Sakhanokho, H.F., P. Ozias-Akins, O.L. May, and P.W. Chee. 2004. Induction of somatic embryogenesis and plant regeneration in select Georgia and Pee Dee cotton (Gossypium hirsutum L.) lines. Crop Science 44:2199-2205. Akiyama, Y., J.A. Conner, S. Goel, D.T. Morishige, J.E. Mullet, W.W. Hanna, and P. Ozias-Akins. 2004. High-resolution physical mapping in Pennisetum squamulatum reveals extensive chromosomal heteromorphism of the genomic region associated with apomixis. Plant Physiol. 134:1733-1741. Yang, H.Y., P. Ozias-Akins, A.K. Culbreath, D.W. Gorbet, J.R. Weeks, B. Mandal, and H.R. Pappu. 2004. Field Evaluation of Tomato spotted wilt virus Resistance in Transgenic Peanut (Arachis hypogaea). Plant Dis. 88:259-264. Goldman, J.J., W.W. Hanna, G.H. Fleming, and P. Ozias-Akins. 2003. Ploidy variation among herbicide-resistant bermudagrass plants of cv TifEagle transformed with the bar gene. Plant Cell Reports 22:553-560. Ozias-Akins, P., Y. Akiyama, and W. W. Hanna. 2003. Molecular characterization of the genomic region linked with apomixis in Pennisetum/Cenchrus. Funct. Integr. Genomics 3:94-104. Goldman, J. J., W. W. Hanna, G. F. Fleming, and P. Ozias-Akins. 2003. Fertile transgenic pearl millet [Pennisetum glaucum (L.) R. Br.] plants recovered through microprojectile bombardment and phosphinothricin selection of apical meristem-, inflorescence-, and immature embryo- derived embryogenic tissues. Plant Cell Rep. 21:999-1009. Yang, H.Y., J. Nairn, and P. Ozias-Akins. 2003. Transformation of peanut using a modified bacterial mercuric ion reductase gene driven by an actin promoter from Arabidopsis thaliana. J. Plant Physiol. 160:945-952. Goel, S., Z. Chen, J.A. Conner, Y. Akiyama, W.W. Hanna, and P. Ozias-Akins. 2003. Physical evidence that a single hemizygous chromosomal region is sufficient to confer aposporous embryo sac formation in Pennisetum squamulatum and Cenchrus ciliaris. Genetics 163:1069-1082. Ozias-Akins, P., H. Yang, R. Gill, H. Fan, R.E. Lynch. 2002. Reduction of aflatoxin contamination in peanut: A genetic engineering approach. ACS Symposium Series 829:151-160. Ozias-Akins, P., R. Gill. 2001. Progress in the development of tissue culture and transformation methods applicable to the production of transgenic peanut. Peanut Sci. 28:123-131. Roche, D.R., Conner, J.A., Budiman, M.A., Frisch, D., Wing, R., Hanna, W.W., Ozias-Akins, P. 2002. Construction of BAC libraries from two apomictic grasses to study the microcolinearity of their apospory-specific genomic regions. Theor. Appl. Genet. 104:804-812. Roche, D., W.W. Hanna, and P. Ozias-Akins. 2001. Gametophytic apomixis, polyploidy, and supernumerary chromatin. Sex. Plant Reprod. 13:343-349. Roche, D.R., Z. Chen, W.W. Hanna, and P. Ozias-Akins. 2001. Non-Mendelian transmission of an apospory-specific genomic region in a reciprocal cross between sexual pearl millet (Pennisetum glaucum) and an apomictic F1 (P. glaucum X P. squamulatum). Sex. Plant Reprod. 13:217-223. Goldman, J.J., P. Ozias-Akins, and W.W. Hanna. 2000. GUS transient gene expression in bombarded pearl millet tissue. International Sorghum and Millets Newsletter 41: 74-76. Gill, R. and P. Ozias-Akins. 1999. Thidiazuron-induced highly morphogenic callus and high frequency regeneration of fertile peanut (Arachis hypogaea L.) plants. In Vitro Cell. Dev. Biol. Plant 35:445-450. Roche, D., P. Cong, Z. Chen, W.W. Hanna, D.L. Gustine, R.T. Sherwood, and P. Ozias-Akins. 1999. An apospory-specific genomic region is conserved between buffelgrass (Cenchrus ciliaris L.) and Pennisetum squamulatum Fresen. Plant J. 19:203-208. Zhang, L.-H., P. Ozias-Akins, G. Kochert, S. Kresovich, R. Dean, and W. Hanna. 1999. Genotype identification of bermudagrass (Cynodon spp.) by AFLP analysis. Theor. Appl. Genet. 98:895-902. Mitchell, T.D., A.S. Bhagsari, P. Ozias-Akins, and S.K. Dhir. 1998. Electroporation-mediated transient gene expression in intact cells of sweetpotato. In Vitro Cell. Dev. Biol. Plant 34:319-324. Ozias-Akins, P., D. Roche, and W.W. Hanna. 1998. Tight clustering and hemizygosity of apomixis-linked molecular markers in Pennisetum squamulatum implies genetic control of apospory by a divergent locus which may have no allelic form in sexual genotypes. Proc. Natl. Acad. Sci. USA 95:5127-5132. Yang, H., C. Singsit, A. Wang, D. Gonsalves, and P. Ozias-Akins. 1998. Transgenic peanut plants containing a nucleocapsid protein gene of tomato spotted wilt virus show divergent levels of gene expression. Plant Cell Rep. 17:693-699. Morgan, R.N., J.P. Wilson, W.W. Hanna, and P. Ozias-Akins. 1998. Molecular markers for rust and pyricularia leaf spot disease resistance in pearl millet. Theor. Appl. Genet. 96:413-420. Wang, A., H. Fan, C. Singsit, and P. Ozias-Akins. 1998.Transformation of peanut with a soybean vspB promoter-uidA chimeric gene I. Optimization of a transformation system and analysis of GUS expression in primary transgenic tissues and plants. Physiol. Plant. 102:38-48. Morgan, R.N., P. Ozias-Akins, and W.W. Hanna. 1998. Seed set in an apomictic BC3 pearl millet. Intl. J. Plant Sci. 159:89-97. Morgan, R.N., J. Alvernaz, L. Arthur, W.W. Hanna, P. Ozias-Akins. 1997. Genetic characterization and floral development of female sterile and stubby head, two aposporous mutants of pearl millet. Sex Plant Reprod. 10:127-135. Karakas, B., P. Ozias-Akins, C. Stushnoff, M. Suefferheld, and M. Rieger. 1997. Salinity and drought tolerance of mannitol accumulating transgenic tobacco. Plant Cell Environ. 20:609-616. Singsit, C., M.J. Adang, R.E. Lynch, W.F. Anderson, A. Wang, G. Cardineau, and P. Ozias-Akins. 1997. Expression of a Bacillus thuringiensis crylA(c) gene in transgenic peanut plants and its efficacy against lesser cornstalk borer. Transgenic Res. 6:169-176. Aruna, M., M.E. Austin, and P. Ozias-Akins. 1995. DNA amplification fingerprinting for identification of rabbiteye blueberry (Vaccinium ashei Reade) cultivars. J. Amer. Soc. Hort. Sci. 120:710-713. Jarret, R.L., P. Ozias-Akins, S. Phatak, R. Nadimpali, S. Hilliard. 1995. DNA contents in Paspalum spp. determined by flow cytometry. J. Genet. Res. Crop Evol. 42:237-242. Knauft, D.A. and P. Ozias-Akins. 1995. Recent methodologies for germplasm enhancement and breeding. In: Advances in Peanut Science, H.E. Pattee and H.T. Stalker, eds., American Peanut Research and Education Society. Anderson, W.F., L. Arthur, P. Ozias-Akins. 1994. Quick and efficient measurement of transient GUS expression using image analysis. Plant Mol. Biol. Rep. 12:332-340. Lubbers, E.L., L. Arthur, W.W. Hanna, P. Ozias-Akins. 1994. Molecular markers shared by diverse apomictic Pennisetum species. Theor. Appl. Genet. 89:636-642. Ozias-Akins, P. and R.L. Jarret. 1994. Flow cytometric determination of ploidy levels in Ipomoea. J. Amer. Soc. Hort. Sci. 119:110-115. Ozias-Akins, P., J.A. Schnall, W.F. Anderson, C. Singsit, T.E. Clemente, M.J. Adang, and A.K. Weissinger. 1993. Regeneration of transgenic peanut plants from stably transformed embryogenic callus. Plant Science 93:185-194. Aruna, M., P. Ozias-Akins, M.E. Austin, and G. Kochert. 1993. Genetic relatedness among rabbiteye blueberry (Vaccinium ashei) cultivars determined by DNA amplification using single primers of arbitrary sequence. Genome 36:971-977. Singsit, C. and P. Ozias-Akins. 1993. Genetic variation in androgenetic monoploids of diploid potato and identification of genome-specific random amplified polymorphic DNA markers. Plant Cell Rep. 12:144-148. Hanna, W.W., M. Dujardin, P. Ozias-Akins, E.L. Lubbers, and L. Arthur. 1993. Reproduction, cytology and fertility of pearl millet x Pennisetum squamulatum BC4 plants. J. Hered. 84:213-216. Ozias-Akins, P., E.L. Lubbers, W.W. Hanna, and J.W. McNay. 1993. Transmission of the apomictic mode of reproduction in Pennisetum: Co-inheritance of the trait and molecular markers. Theor. Appl. Genet. 85:632-638. Arthur, L., P. Ozias-Akins, and W.W. Hanna. 1993. Female sterile mutant in pearl millet - evidence for initiation of apospory. J. Hered. 84:112-115. Singsit, C. and P. Ozias-Akins. 1992. Rapid estimation of ploidy levels in in vitro-regenerated Arachis hybrids and fertile triploids. Euphytica 64:183-188. Ozias-Akins, P., W.F. Anderson, and C.C. Holbrook. 1992. Somatic embryogenesis in Arachis hypogaea L.: Genotype comparison. Plant Sci. 83:103-111. Ozias-Akins, P., C. Singsit, and W.D. Branch. 1992. Interspecific hybrid inviability in crosses of Arachis hypogaea x A. stenosperma can be overcome by in vitro embryo maturation or somatic embryogenesis. J. Plant Physiol. 140:207-212. Kvien, C.K. and P. Ozias-Akins. 1991. Lack of monocarpic senescence in Florunner peanut. Peanut Sci. 19:86-90. Perera, S.C., and P. Ozias-Akins. 1991. Regeneration from sweetpotato protoplasts and assessment of growth conditions for flow-sorting of fusion mixtures. J. Amer. Soc. Hort. Sci. 116:917-922. Ozias-Akins, P. 1989. Plant regeneration from immature embryos of peanut. Plant Cell Rep. 8:217-218. Ozias-Akins, P., M. Dujardin, W.W. Hanna, I.K. Vasil. 1989. Quantitative variation recovered from tissue cultures of an apomictic, interspecific Pennisetum hybrid. Maydica 34:123-132. Ozias-Akins, P. and I.K. Vasil. 1988. In vitro regeneration and genetic manipulation of grasses. Physiol. Plant. 73:565-569. Hauptmann, R.M., V. Vasil, P. Ozias-Akins, Z. Tabaeizadeh, S.G. Rogers, R.T. Fraley, R.B. Horsch, I.K. Vasil. 1988. Evaluation of selectable markers for obtaining stable transformants in the Gramineae. Plant Physiol. 86:602-606. Ozias-Akins, P., Z. Tabaeizadeh, D.R. Pring, I.K. Vasil. 1988. Preferential amplification of mitochondrial DNA fragments in somatic hybrids of the Gramineae. Curr. Genet. 13:241-245. Hauptmann, R.M., P. Ozias-Akins, V. Vasil, Z. Tabaeizadeh, S.G. Rogers, R.B. Horsch, I.K. Vasil, R.T. Fraley. 1987. Transient expression of electroporated DNA in monocotyledonous and dicotyledonous species. Plant Cell Rep. 6:265-270. Ozias-Akins, P., D.R. Pring, I.K. Vasil. 1987. Rearrangements in the mitochondrial genome of somatic hybrid cell lines of Pennisetum americanum (L.) K. Schum. + Panicum maximum. Jacq. Theor. Appl. Genet. 74:15-20. Ozias-Akins, P., R.J. Ferl, I.K. Vasil. 1986. Somatic hybridization in the Gramineae: Pennisetum americanum + Panicum maximum. Mol. Gen. Genet. 203:365-370. Rao, P.S. and P. Ozias-Akins. 1985. Plant regeneration through somatic embryogenesis in protoplast cultures of sandalwood (Santalum album L.). Protoplasma 124:80-86. Ozias-Akins, P. and H. Lörz. 1984. Progress and limitations in the culture of cereal protoplasts. Trends in Biotech. 2:119-123. Ozias-Akins, P. and I.K. Vasil. 1983. Improved efficiency and normalization of somatic embryogenesis in Triticum aestivum (wheat). Protoplasma 117:40-44. Ozias-Akins, P. and I.K. Vasil. 1983. Proliferation of and plant regeneration from the epiblast of Triticum aestivum L. (wheat). Amer. J. Bot. 70:1092-1097. Ozias-Akins, P. and I.K. Vasil. 1982. Callus induction and growth from the mature embryo of Triticum aestivum L. (wheat). Protoplasma 115:104-113. Lu, C., I.K. Vasil, and P. Ozias-Akins. 1982. Somatic embryogenesis in Zea mays L. Theor. Appl. Genet. 62:109-112. Pence, V.C., N.J. Novick, P. Ozias-Akins, and I.K. Vasil. 1982. Induction of nitrogenase activity in Azospirillum brasilense by conditioned medium from suspension cultures of Pennisetum americanum (pearl millet) and Panicum maximum (Guinea grass). Z. Pflanzenphysiol. 106:139-147. Ozias-Akins, P. and I.K. Vasil 1982. Plant regeneration from cultured immature embryos and inflorescences of Triticum aestivum L. (wheat): Evidence for somatic embryogenesis. Protoplasma 110:95-105. |
| back to top | contact information Peggy Ozias-Akins, Lab PI, Professor, Department of Horticulture and NESPAL, The University of Georgia, Tifton Campus email pozias@uga.edu • phone (229) 386-3902 • fax (229) 386-7371 Billing Address • P.O. Box 748, Tifton, GA 31793-0748 Physical Address • 2356 Rainwater Road, Tifton, GA 31794 |