Ozias-Akins Biotechnology Laboratory
papers & presentations
Selected Papers and Presentations
View selected papers and presentations listed on the NESPAL website.

All Refereed Publications
Akiyama, Y, S. Goel, Z. Chen, W.W. Hanna, and P. Ozias-Akins. 2006. Pennisetum squamulatum: Is the Predominant Cytotype Hexaploid or Octaploid? J. Hered. doi:10.1093/jhered/esl005

Gualtieri, G., J. A. Conner, D. T. Morishige, J. E. Mullet, and P. Ozias-Akins. 2006. A segment of the apospory-specific genomic region is highly microsyntenic not only between the apomicts Pennisetum squamulatum and buffelgrass, but also with a rice chromosome 11 centromeric-proximal genomic region.  Plant Physiol. 140:963-971.

Goel, S., Z. Chen, Y. Akiyama, J. A. Conner, M. Basu, G. Gualtieri, W. W. Hanna, P. Ozias-Akins. 2006. Comparative Physical Mapping of the Apospory-Specific Genomic Region in Two Apomictic Grasses, Pennisetum squamulatum and Cenchrus ciliaris. Genetics 173:389-400.

Ramos, M.L., G. Fleming, Y. Chu, Y. Akiyama, M.Gallo, P. Ozias-Akins. 2006. Genomic and evolutionary context for conglutin genes in Arachis. Mol. Gen. Gen. 275:578-592.

Ozias-Akins, P.  2006.  Apomixis: Developmental Characteristics and Genetics. Crit. Rev. Plant Sci. 25:199-214.

Ozias-Akins, P.  2005.  Peanut.  In: Biotechnology in Agriculture and Forestry – Tropical Crops I. E.C. Pua and M.R. Davey, eds.  Springer, Heidelberg.

Sorghum Genomics Planning Workshop Participants. 2005. Toward Sequencing the Sorghum Genome. A U.S. National Science Foundation-Sponsored Workshop Report. Plant Physiol. 138:1898-1902.

Akiyama, Y., W.W. Hanna, P. Ozias-Akins. 2005. High-resolution mapping reveals that the apospory-specific genomic region (ASGR) in C. ciliaris is located on a heterochromatic and hemizygous region of a single chromosome. Theor. Appl. Genet. 111:1042-1051.

Joshi, M., C. Niu, G. Fleming, S. Hazra, Y. Chu, C. J. Nairn, H. Yang, P. Ozias-Akins.  2005.  Use of green fluorescent protein as a non-destructive marker for peanut genetic transformation .  In Vitro Plant 41:437-445.

Sakhanokho, H.F., P. Ozias-Akins, O.L. May, and P.W. Chee. 2005. Putrescine enhances somatic embryogenesis and plant regeneration in elite upland cotton (Gossypium hirsutum L.) genotypes. Plant Cell Tiss. Organ Cult. 81:91-95.

Jauhar, P.P., K.N. Rai, P. Ozias-Akins, Z. Chen, and W.W. Hanna. 2005. Genetic Improvement of Pearl Millet for Grain and Forage Production: Cytogenetic Manipulation and Heterosis Breeding.  In: R.J. Singh and P.P. Jauhar, eds., Genetic Resources, Chromosome Engineering and Crop Improvement, CRC Taylor and Francis, pp 281-307.

Goldman, J. W.W. Hanna, and P. Ozias-Akins.  2004.  Tissue culture, plant regeneration and an analysis of somaclonal variation from TifEagle and TifSport bermudagrass cultivars.  HortScience 39:1381-1384.

Sakhanokho, H.F., P. Ozias-Akins, O.L. May, and P.W. Chee. 2004. Induction of somatic embryogenesis and plant regeneration in select Georgia and Pee Dee cotton (Gossypium hirsutum L.) lines. Crop Science 44:2199-2205.

Akiyama, Y., J.A. Conner, S. Goel, D.T. Morishige, J.E. Mullet, W.W. Hanna, and P. Ozias-Akins. 2004. High-resolution physical mapping in Pennisetum squamulatum reveals extensive chromosomal heteromorphism of the genomic region associated with apomixis.  Plant Physiol. 134:1733-1741.

Yang, H.Y., P. Ozias-Akins, A.K. Culbreath, D.W. Gorbet, J.R. Weeks, B. Mandal, and H.R. Pappu. 2004.  Field Evaluation of Tomato spotted wilt virus Resistance in Transgenic Peanut (Arachis hypogaea).  Plant Dis. 88:259-264.

Goldman, J.J., W.W. Hanna, G.H. Fleming, and P. Ozias-Akins. 2003.  Ploidy variation among herbicide-resistant bermudagrass plants of cv TifEagle transformed with the bar gene.  Plant Cell Reports 22:553-560.

Ozias-Akins, P., Y. Akiyama, and W. W. Hanna. 2003. Molecular characterization of the genomic region linked with apomixis in Pennisetum/Cenchrus.  Funct. Integr. Genomics 3:94-104.

Goldman, J. J., W. W. Hanna, G. F. Fleming, and P. Ozias-Akins.  2003.  Fertile transgenic pearl millet [Pennisetum glaucum (L.) R. Br.] plants recovered through microprojectile bombardment and phosphinothricin selection of apical meristem-, inflorescence-, and immature embryo- derived embryogenic tissues. Plant Cell Rep. 21:999-1009.

Yang, H.Y., J. Nairn, and P. Ozias-Akins. 2003. Transformation of peanut using a modified bacterial mercuric ion reductase gene driven by an actin promoter from Arabidopsis thaliana.  J. Plant Physiol. 160:945-952.

Goel, S., Z. Chen, J.A. Conner, Y. Akiyama, W.W. Hanna, and P. Ozias-Akins. 2003. Physical evidence that a single hemizygous chromosomal region is sufficient to confer aposporous embryo sac formation in Pennisetum squamulatum and Cenchrus ciliaris.  Genetics 163:1069-1082.

Ozias-Akins, P., H. Yang, R. Gill, H. Fan, R.E. Lynch. 2002. Reduction of aflatoxin contamination in peanut: A genetic engineering approach.  ACS Symposium Series 829:151-160.

Ozias-Akins, P., R. Gill.  2001.  Progress in the development of tissue culture and transformation methods applicable to the production of transgenic peanut.  Peanut Sci. 28:123-131.

Roche, D.R., Conner, J.A., Budiman, M.A., Frisch, D., Wing, R., Hanna, W.W., Ozias-Akins, P. 2002. Construction of BAC libraries from two apomictic grasses to study the microcolinearity of their apospory-specific genomic regions.  Theor. Appl. Genet. 104:804-812.

Roche, D., W.W. Hanna, and P. Ozias-Akins.  2001.  Gametophytic apomixis, polyploidy, and supernumerary chromatin.  Sex. Plant Reprod. 13:343-349.

Roche, D.R., Z. Chen, W.W. Hanna, and P. Ozias-Akins.  2001.  Non-Mendelian transmission of an apospory-specific genomic region in a reciprocal cross between sexual pearl millet (Pennisetum glaucum) and an apomictic F1 (P. glaucum X P. squamulatum).  Sex. Plant Reprod. 13:217-223.

Goldman, J.J., P. Ozias-Akins, and W.W. Hanna. 2000. GUS transient gene expression in bombarded pearl millet tissue. International Sorghum and Millets Newsletter 41: 74-76.

Gill, R. and P. Ozias-Akins.  1999. Thidiazuron-induced highly morphogenic callus and high frequency regeneration of fertile peanut (Arachis hypogaea L.) plants.  In Vitro Cell. Dev. Biol. Plant 35:445-450.

Roche, D., P. Cong, Z. Chen, W.W. Hanna, D.L. Gustine, R.T. Sherwood, and P. Ozias-Akins. 1999. An apospory-specific genomic region is conserved between buffelgrass (Cenchrus ciliaris L.) and Pennisetum squamulatum Fresen. Plant J. 19:203-208.

Zhang, L.-H., P. Ozias-Akins, G. Kochert, S. Kresovich, R. Dean, and W. Hanna. 1999. Genotype identification of bermudagrass (Cynodon spp.) by AFLP analysis. Theor. Appl. Genet. 98:895-902.

Mitchell, T.D., A.S. Bhagsari, P. Ozias-Akins, and S.K. Dhir. 1998. Electroporation-mediated transient gene expression in intact cells of sweetpotato. In Vitro Cell. Dev. Biol. Plant 34:319-324.

Ozias-Akins, P., D. Roche, and W.W. Hanna. 1998. Tight clustering and hemizygosity of apomixis-linked molecular markers in Pennisetum squamulatum implies genetic control of apospory by a divergent locus which may have no allelic form in sexual genotypes. Proc. Natl. Acad. Sci. USA 95:5127-5132.

Yang, H., C. Singsit, A. Wang, D. Gonsalves, and P. Ozias-Akins.  1998.  Transgenic peanut plants containing a nucleocapsid protein gene of tomato spotted wilt virus show divergent levels of gene expression.  Plant Cell Rep. 17:693-699.

Morgan, R.N., J.P. Wilson, W.W. Hanna, and P. Ozias-Akins.  1998.  Molecular markers for rust and pyricularia leaf spot disease resistance in pearl millet.  Theor. Appl. Genet. 96:413-420.

Wang, A., H. Fan, C. Singsit, and P. Ozias-Akins. 1998.Transformation of peanut with a soybean vspB promoter-uidA chimeric gene  I. Optimization of a transformation system and analysis of GUS expression in primary transgenic tissues and plants. Physiol. Plant. 102:38-48.

Morgan, R.N., P. Ozias-Akins, and W.W. Hanna. 1998. Seed set in an apomictic BC3 pearl millet. Intl. J. Plant Sci. 159:89-97.

Morgan, R.N., J. Alvernaz, L. Arthur, W.W. Hanna, P. Ozias-Akins. 1997. Genetic characterization and floral development of female sterile and stubby head, two aposporous mutants of pearl millet. Sex Plant Reprod. 10:127-135.

Karakas, B., P. Ozias-Akins, C. Stushnoff, M. Suefferheld, and M. Rieger. 1997. Salinity and drought tolerance of mannitol accumulating transgenic tobacco. Plant Cell Environ. 20:609-616.

Singsit, C., M.J. Adang, R.E. Lynch, W.F. Anderson, A. Wang, G. Cardineau, and P. Ozias-Akins. 1997. Expression of a Bacillus thuringiensis crylA(c) gene in transgenic peanut plants and its efficacy against lesser cornstalk borer.  Transgenic Res. 6:169-176.

Singsit, C., C.C. Holbrook, A.K. Culbreath, and P. Ozias-Akins. 1995. Progenies of an interspecific hybrid between Arachis hypogaea and A. stenosperma - pest resistance and molecular homogeneity. Euphytica 83:9-14.

Aruna, M., M.E. Austin, and P. Ozias-Akins. 1995. DNA amplification fingerprinting for identification of rabbiteye blueberry (Vaccinium ashei Reade) cultivars. J. Amer. Soc. Hort. Sci. 120:710-713.

Jarret, R.L., P. Ozias-Akins, S. Phatak, R. Nadimpali, S. Hilliard. 1995. DNA contents in Paspalum spp. determined by flow cytometry. J. Genet. Res. Crop Evol. 42:237-242.

Knauft, D.A. and P. Ozias-Akins.  1995.  Recent methodologies for germplasm enhancement and breeding.  In:  Advances in Peanut Science, H.E. Pattee and H.T. Stalker, eds., American Peanut Research and Education Society.

Anderson, W.F., L. Arthur, P. Ozias-Akins. 1994. Quick and efficient measurement of transient GUS expression using image analysis. Plant Mol. Biol. Rep. 12:332-340.

Lubbers, E.L., L. Arthur, W.W. Hanna, P. Ozias-Akins. 1994. Molecular markers shared by diverse apomictic Pennisetum species. Theor. Appl. Genet. 89:636-642.

Ozias-Akins, P. and R.L. Jarret.  1994.  Flow cytometric determination of ploidy levels in Ipomoea.  J. Amer. Soc. Hort. Sci. 119:110-115.

Ozias-Akins, P., J.A. Schnall, W.F. Anderson, C. Singsit, T.E. Clemente, M.J. Adang, and A.K. Weissinger.  1993.  Regeneration of transgenic peanut plants from stably transformed embryogenic callus.  Plant Science 93:185-194.

Aruna, M., P. Ozias-Akins, M.E. Austin, and G. Kochert.  1993.  Genetic relatedness among rabbiteye blueberry (Vaccinium ashei) cultivars determined by DNA amplification using single primers of arbitrary sequence.  Genome 36:971-977.

Singsit, C. and P. Ozias-Akins.  1993.  Genetic variation in androgenetic monoploids of diploid potato and identification of genome-specific random amplified polymorphic DNA markers.  Plant Cell Rep. 12:144-148.

Hanna, W.W., M. Dujardin, P. Ozias-Akins, E.L. Lubbers, and L. Arthur.  1993. Reproduction, cytology and fertility of pearl millet x Pennisetum squamulatum BC4 plants.  J. Hered. 84:213-216.

Ozias-Akins, P., E.L. Lubbers, W.W. Hanna, and J.W. McNay. 1993. Transmission of the apomictic mode of reproduction in Pennisetum: Co-inheritance of the trait and molecular markers.  Theor. Appl. Genet. 85:632-638.

Arthur, L., P. Ozias-Akins, and W.W. Hanna.  1993.  Female sterile mutant in pearl millet - evidence for initiation of apospory.  J. Hered. 84:112-115.

Singsit, C. and P. Ozias-Akins.  1992.  Rapid estimation of ploidy levels in in vitro-regenerated Arachis hybrids and fertile triploids.  Euphytica 64:183-188.

Ozias-Akins, P., W.F. Anderson, and C.C. Holbrook. 1992. Somatic embryogenesis in Arachis hypogaea L.: Genotype comparison. Plant Sci. 83:103-111.

Ozias-Akins, P., C. Singsit, and W.D. Branch. 1992. Interspecific hybrid inviability in crosses of Arachis hypogaea x A. stenosperma can be overcome by in vitro embryo maturation or somatic embryogenesis. J. Plant Physiol. 140:207-212.

Kvien, C.K. and P. Ozias-Akins. 1991. Lack of monocarpic senescence in Florunner peanut. Peanut Sci. 19:86-90.

Perera, S.C., and P. Ozias-Akins. 1991. Regeneration from sweetpotato protoplasts and assessment of growth conditions for flow-sorting of fusion mixtures. J. Amer. Soc. Hort. Sci. 116:917-922.

Ozias-Akins, P. 1989. Plant regeneration from immature embryos of peanut. Plant Cell Rep. 8:217-218.

Ozias-Akins, P., M. Dujardin, W.W. Hanna, I.K. Vasil. 1989. Quantitative variation recovered from tissue cultures of an apomictic, interspecific Pennisetum hybrid. Maydica 34:123-132.

Ozias-Akins, P. and I.K. Vasil. 1988. In vitro regeneration and genetic manipulation of grasses. Physiol. Plant. 73:565-569.

Hauptmann, R.M., V. Vasil, P. Ozias-Akins, Z. Tabaeizadeh, S.G. Rogers, R.T. Fraley, R.B. Horsch, I.K. Vasil. 1988. Evaluation of selectable markers for obtaining stable transformants in the Gramineae. Plant Physiol. 86:602-606.

Ozias-Akins, P., Z. Tabaeizadeh, D.R. Pring, I.K. Vasil. 1988. Preferential amplification of mitochondrial DNA fragments in somatic hybrids of the Gramineae.  Curr. Genet. 13:241-245.

Hauptmann, R.M., P. Ozias-Akins, V. Vasil, Z. Tabaeizadeh, S.G. Rogers, R.B. Horsch, I.K. Vasil, R.T. Fraley. 1987. Transient expression of electroporated DNA in monocotyledonous and dicotyledonous species. Plant Cell Rep. 6:265-270.

Ozias-Akins, P., D.R. Pring, I.K. Vasil. 1987. Rearrangements in the mitochondrial genome of somatic hybrid cell lines of Pennisetum americanum (L.) K. Schum. + Panicum maximum. Jacq. Theor. Appl. Genet. 74:15-20.

Ozias-Akins, P., R.J. Ferl, I.K. Vasil. 1986. Somatic hybridization in the Gramineae:  Pennisetum americanum + Panicum maximum. Mol. Gen. Genet. 203:365-370.

Rao, P.S. and P. Ozias-Akins. 1985. Plant regeneration through somatic embryogenesis in protoplast cultures of sandalwood (Santalum album L.). Protoplasma 124:80-86.

Ozias-Akins, P. and H. Lörz.  1984.  Progress and limitations in the culture of cereal protoplasts. Trends in Biotech. 2:119-123.

Ozias-Akins, P. and I.K. Vasil. 1983. Improved efficiency and normalization of somatic embryogenesis in Triticum aestivum (wheat). Protoplasma 117:40-44.

Ozias-Akins, P. and I.K. Vasil. 1983. Proliferation of and plant regeneration from the epiblast of Triticum aestivum L. (wheat). Amer. J. Bot. 70:1092-1097.

Ozias-Akins, P. and I.K. Vasil. 1982. Callus induction and growth from the mature embryo of Triticum aestivum L. (wheat). Protoplasma 115:104-113.

Lu, C., I.K. Vasil, and P. Ozias-Akins. 1982. Somatic embryogenesis in Zea mays L. Theor. Appl. Genet. 62:109-112.

Pence, V.C., N.J. Novick, P. Ozias-Akins, and I.K. Vasil. 1982. Induction of nitrogenase activity in Azospirillum brasilense by conditioned medium from suspension cultures of Pennisetum americanum (pearl millet) and Panicum maximum (Guinea grass). Z. Pflanzenphysiol. 106:139-147.

Ozias-Akins, P. and I.K. Vasil 1982. Plant regeneration from cultured immature embryos and inflorescences of Triticum aestivum L. (wheat):  Evidence for somatic embryogenesis. Protoplasma 110:95-105.

contact information
Peggy Ozias-Akins, Lab PI, Professor,
Department of Horticulture and NESPAL, The University of Georgia, Tifton Campus
email pozias@uga.edu • phone (229) 386-3902 • fax (229) 386-7371

Billing Address • P.O. Box 748, Tifton, GA 31793-0748
Physical Address • 2356 Rainwater Road, Tifton, GA 31794